bj normal human foreskin primary fibroblast cell line  (ATCC)

Journal: Scientific Reports

Article Title: Chitosan-dextran sulfate nanocapsules for enhanced tigecycline efficacy against non-typhoidal Salmonella enterica

doi: 10.1038/s41598-026-35229-7

Figure Lengend Snippet: TGC and/or unloaded CD NPs ameliorated the S. Typhimurium infected group induced histopathological alterations in mice’s liver tissues. Representative photomicrographs of the H&E-stained hepatic tissue sections showing the control (A), S. Typhimurium infected group (C), TGC (E), unloaded CD NPs (G), CD-TGC groups (I) and their respective higher magnifications (B, D, F, H, and J). A, B: control group displaying normal central vein (CV), hepatic cords (HC) with hepatocytes of eosinophilic granular cytoplasm (EC), rounded central single (SN) or double vesicular nuclei (DN), and kupffer cells (KC). C, D: S. Typhimurium infected group demonstrating multiple areas of variable-sized necrotic areas (NA) of coagulative necrosis (CN), severely dilated and congested sinusoid (SDS) with Kupffer cell hyperplasia (KCH). E, F: TGC group displaying moderately sized necrotic areas (MNA), moderately dilated and congested sinusoids (MDS), moderately hyperplastic Kupffer’s cells (MKC), interstitial mononuclear cell infiltration (MI), fibroblast proliferation (FP), and regenerated hepatocytes of stippling basophilic cytoplasm (BC) and pale nuclei (PN). G, H: unloaded CD NPs group showing a few scattered minute necrotic areas (mNA), intense mononuclear cell infiltration (IMI) around the portal area, and apparently normal hepatocytes (NH). I, J: CD-TGC group showed normal hepatocytes (NH), few dilated blood vessels (DBV), and a few interstitial lymphocytic aggregates (FL). Scale bars = 100 μm in A, C, E, G, I; and = 20 μm in B, D, F, H, and J. K: Bar charts demonstrate the statistical analysis of the comparative quantification of the hepatic injury scores in all studied groups. Bars carrying different superscript letters (a, b, c, d, and e) are significantly different as analyzed by the one-way ANOVA test, followed by the multiple comparisons by Duncan’s Post-hoc test ( p < 0.05). Values are the mean of 6 mice per group ± S.E.M.

Article Snippet: BJ normal human foreskin primary fibroblast cell line (ATCC CRL-2522) was used for studying safety of CD-TGC nanocapsules.

Techniques: Infection, Staining, Control

Journal: Cell Journal (Yakhteh)

Article Title: The Synergistic Effect of Cold Atmospheric Plasma Mediated Gold Nanoparticles Conjugated with Indocyanine Green as An Innovative Approach to Cooperation with Radiotherapy

doi: 10.22074/CELLJ.2022.559078.1097

Figure Lengend Snippet: MTT assay results on melanoma (DFW) and fibroblast (HFF) cell lines at 24 hours. A. The survival rate of DFW and HFF cells incubated with different concentrations of GNP@ICG in 24 hours. The concentration of 10 μM which caused 10% cell death was selected for following treatments. B. The survival percentage of cells treated with radiotherapy. C. The survival percentage of cells treated with CAP. D. The viability of the cells treated to 2 Gy RT following NPs and CAP (30 and 60 seconds) immediately and 24 hours later. Data are shown as the mean ± SD (n=3). A comparison of the difference between each group and the control group was presented as *; P<0.05, **; P<0.01, and ***; P<0.001. CAP; Cold atmospheric plasma, NPs; GNP@ICG, RT; Radiotherapy, 30s+2Gy/0h; Combined therapy 30-seconds CAP and 2 Gy radiation immediately, 30s+2Gy/24h; Ccombined therapy 30-seconds CAP and 2 Gy radiation with 24 hours interval, 60 s+2Gy/0h; Combined therapy 60-seconds CAP and 2 Gy radiation immediately, and 60s+2Gy/24h; Combined therapy 60-seconds CAP and 2 Gy radiation with 24 hours interval.

Article Snippet: DFW (human melanoma cell line, C496) and HFF (normal human foreskin fibroblast cell line, C163) were purchased from Pasteur Institute in Iran.

Techniques: MTT Assay, Incubation, Concentration Assay

Journal: Cell Journal (Yakhteh)

Article Title: The Synergistic Effect of Cold Atmospheric Plasma Mediated Gold Nanoparticles Conjugated with Indocyanine Green as An Innovative Approach to Cooperation with Radiotherapy

doi: 10.22074/CELLJ.2022.559078.1097

Figure Lengend Snippet: Flow cytometry results of DFW and HFF cell lines. A. The Annexin-V-FITC assay for detecting the apoptosis and necrosis of DFW cells, B. The diagram of the percentage of apoptotic and necrotic of DFW cells after treatment with different groups, C. The Annexin-V-FITC assay for detecting the apoptosis and necrosis of HFF cells, and D. The diagram of percentage of apoptotic and necrotic of HFF cells in different groups. DFW; Melanoma cell line, HFF; Health foreskin fibroblast, CAP; Cold atmospheric plasma, NPs; GNP@ICG, RT; Radiotherapy, 30s+2Gy/0h; Combined therapy 30-seconds CAP and 2 Gy radiation immediately, 30s+2Gy/24h; Ccombined therapy 30-seconds CAP and 2 Gy radiation with 24 hours interval, 60 s+2Gy/0h; Combined therapy 60-seconds CAP and 2 Gy radiation immediately, and 60s+2Gy/24h; Combined therapy 60-seconds CAP and 2 Gy radiation with 24 hours interval.

Article Snippet: DFW (human melanoma cell line, C496) and HFF (normal human foreskin fibroblast cell line, C163) were purchased from Pasteur Institute in Iran.

Techniques: Flow Cytometry

Journal: Nutrients

Article Title: Eriodictyol Attenuates H 2 O 2 -Induced Oxidative Damage in Human Dermal Fibroblasts through Enhanced Capacity of Antioxidant Machinery

doi: 10.3390/nu14122553

Figure Lengend Snippet: Eriodictyol inhibited H 2 O 2 -induced cytotoxicity in dermal fibroblasts. ( A ) H 2 O 2 exposure (125–1000 µM; 1 h) resulted in a concentration-dependent decrease in the viability of BJ cells. ( B ) Eriodictyol treatment (10–40 µM; 24 h) did not result in cytotoxicity in BJ cells. ( C ) Eriodictyol pretreatment (10–40 µM; 24 h) prevented H 2 O 2 -induced cytotoxicity (500 µM; 1 h) in BJ cells. Cell viability was observed using the MTT assay after the indicated treatments. The survival percentage was calculated relative to untreated controls. DMSO (0.5%) was used as a vehicle control ( n = 3; each biological replicate consisted of four independent wells; mean ± SEM). * p < 0.05 versus vehicle controls ( A ) and p < 0.05 versus H 2 O 2 -treated cells ( C ). N.S. , not significant.

Article Snippet: Normal human foreskin fibroblast cell line BJ (ATCC No. CRL-2522) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Concentration Assay, MTT Assay, Control

Journal: Nutrients

Article Title: Eriodictyol Attenuates H 2 O 2 -Induced Oxidative Damage in Human Dermal Fibroblasts through Enhanced Capacity of Antioxidant Machinery

doi: 10.3390/nu14122553

Figure Lengend Snippet: Eriodictyol prevented the induction of necrosis in BJ cells following H 2 O 2 treatment. BJ cells were pre-treated with eriodictyol (5–20 µM; 24 h) followed by H 2 O 2 treatment (500 µM; 1h). After 24 h treatment with H 2 O 2 , dermal fibroblasts were co-stained with Hoechst 33342 (blue)/PI (red) to characterize mode of cell death ( n = 3; magnification = 20×; scale bar = 100 µm).

Article Snippet: Normal human foreskin fibroblast cell line BJ (ATCC No. CRL-2522) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Staining